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Drug Names
Generic Name:Human anti-SARS-CoV2(S-RBD) (Omicron,B. 1. 1.529) IgM ELISA Kit
Principle
This kit was based on indirect enzyme-linked immune-sorbent assay technology. Recombinant nucleocapsid protein was pre-coated onto 96-well plates. And the HRP conjugated antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
Recovery
Matrices listed below were spiked with certain level of anti-SARS-CoV2(S- RBD) (Omicron,B . 1 . 1 .529)IgM and the recovery rates were calculated by comparing the measured value to the expected amount of anti-SARS-CoV2(S- RBD) (Omicron,B . 1 . 1 .529)IgM in samples.
Matrix
Recovery Range (%)
Average (%)
Serum(n=5)
89- 104
97
EDTA Plasma(n=5)
89-99
95
Heparin Plasma(n=5)
86- 101
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of anti-SARS-CoV2(S- RBD) (Omicron,B . 1 . 1 .529)IgM and their serial dilutions. The results were demonstrated by percentage of calculated concentration to the expectation.
Sample
1:2
1:4
1:8
Serum(n=5)
87-98%
88- 100%
81-94%
EDTA Plasma(n=5)
85- 100%
83- 100%
83- 100%
Heparin Plasma(n=5)
85- 100%
84-99%
88- 100%
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
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