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Human Anti-2019 nCov (N+S) IgG ELISA Kit with positive control
Human Anti-2019 nCov (N+S) IgG ELISA Kit with positive control
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  • NO.:CSL3881Hu
    Sample Volume:10ul
    Wavelength:450nm
    Assay range:3.906-250ng/ml
    Sensitivity:2.344ng/ml
    Method:Indirect Elisa
  • Goods click count:134
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Details

Drug Names

Generic NameHuman anti-2019 nCoV(N+S) IgG ELISA Kit

Principle

This kit was based on indirect enzyme-linked immune-sorbent assay technology. Recombinant nucleocapsid protein was pre-coated onto 96-well plates. And the HRP conjugated antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

Sequence (antigen)

MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQ QSMSSADSTQA

Item

Specifications(96T)

Storage

ELISA Microplate(coated with nCov(N+S))

8×12

2-8°C/-20°C

ELISA Microplate(coated with albumin)

8×12

2-8°C/-20°C

Standard(Positive control)

1 vial

2-8°C

Sample/Standard Dilution Buffer

20ml

2-8°C

HRP-labeled Antibody(Concentrated)

60ul

2-8°C(Avoid Direct Light)

Antibody Dilution Buffer

5ml

2-8°C

TMB Substrate

5ml

2-8°C(Avoid Direct Light)

Stop Solution

5ml

2-8°C

Wash Buffer(25X)

30ml

2-8°C

Plate Sealer

5pieces

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