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Drug Names
Generic Name: Human Phosphorylated Microtubule Associated Protein Tau (pMAPT/pTAU) ELISA Kit
Purpose
Our Human Phosphorylated Microtubule Associated Protein Tau (pMAPT/pTAU) ELISA kit is to assay pMAPT/pTAU levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to pMAPT/pTAU. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for pMAPT/pTAU is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain pMAPT/pTAU and HRP conjugated pMAPT/pTAU antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of pMAPT/pTAU. You can calculate the concentration of pMAPT/pTAU in the samples by comparing the OD of the samples to the standard curve.
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