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Drug Names
Generic Name: Human EIF2A/EIF2 Alpha ELISA Kit
Purpose
Our Human EIF2A/EIF2 Alpha ELISA kit is to assay EIF2A/EIF2 Alpha levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to EIF2A/EIF2 Alpha. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for EIF2A/EIF2 Alpha is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain EIF2A/EIF2 Alpha and HRP conjugated EIF2A/EIF2 Alpha antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of EIF2A/EIF2 Alpha. You can calculate the concentration of EIF2A/EIF2 Alpha in the samples by comparing the OD of the samples to the standard curve.
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