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Drug Names
Generic Name: Human Cytochrome P450 2C19 (CYP2C19) ELISA Kit
Purpose
Our Human Cytochrome P450 2C19 (CYP2C19) ELISA kit is to assay CYP2C19 levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to CYP2C19, Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for CYP2C19 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain CYP2C19 and HRP conjugated CYP2C19 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of CYP2C19. You can calculate the concentration of CYP2C19 in the samples by comparing the OD of the samples to the standard curve.
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