Drug Names

Generic Name: Human B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) ELISA Kit

Purpose

Our Human B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) ELISA kit is to assay Bcl2L levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Bcl2L, Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Bcl2L is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Bcl2L and HRP conjugated Bcl2L antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Bcl2L. You can calculate the concentration of Bcl2L in the samples by comparing the OD of the samples to the standard curve.

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