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Drug Names
Generic Name: Human Histatin 3 (HTN3) ELISA Kit
Purpose
Our Human Histatin 3 (HTN3) ELISA kit is to assay HTN3 levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to HTN3. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for HTN3 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HTN3 and HRP conjugated HTN3 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HTN3. You can calculate the concentration of HTN3 in the samples by comparing the OD of the samples to the standard curve.
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