NADKinase (NADK) Activity Assay Kit

Note: Take two or three different samples for prediction before the formal determination. Detection equipment:Spectrophotometer

Cat No:AK0500

Size:50T/24S

Description:

NAD Kinase(NADK, EC 2.7.1.23) is widely found in animals, plants, microorganisms and cultured cells. It is the only enzyme that can catalyzes the phosphorylation of NAD+  to NADP+  in organisms. It can catalyzethe phosphorylation reaction of NAD(H) with ATP or inorganic polyphosphate [poly(P)] as a phosphoryl donor to generate NADP(H). Therefore, NAD kinase plays an important role in the synthesis of NADP(H) and the regulation of the balance between NAD(H) and NADP(H).

NADK catalyzes the phosphorylation of NAD+ to produce NADP+,which can be reduced to NADPH by

glucose-6-phosphate  dehydrogenase.NADPH  could reduce  oxidized thiazole blue  (MTT) through the dehydrogenation of PMS. The absorbance value of MTT at 570 nm can be reflect the activity of NADK.

 

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