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NAD Malic Enzyme(NAD-ME) Activity Assay Kit
Note: Take two or three different samples for prediction before test.
Detection equipment:Spectrophotometer/Microplate reader
Cat No:AK0483
Size:100T/96S
Description:
MEwidely exists in microbe, culturing cells, animal and plantcytoplasm. Especially it has high activity in plant tissue. ME catalyzesthe reversible reaction of malic acidoxidative decarboxylation to produce pyruvic acid and CO2with the reduction of NAD(P)+, which is the crucial enzyme of malic acid metabolism. The activity of ME is related with biosynthesis and anti-oxidation. In recent years, the determination of plant ME activity become a hot spot in antioxidant research. According the coenzyme specificity and substrate specificity, ME is divided into NAD-ME(EC1. 1. 1.38) and NADP- ME(EC 1.1.1.40).
NADP-ME catalyzesthe reduction of NADP+ to NADPH,detect the increase rate of NADPH at 340nm.
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