Thioredoxin Reductase (TrxR) Activity Assay Kit

Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer

Cat No: AK0482

Size: 50T/48S

Product Description:

Thioredoxin  Reductase  (TrxR)  is  a  NADPH-dependent  dimer  selenase  and  includes  FAD  structure domain. TrxR belongs to pyridine nucleotide-disulfide REDOX enzyme, and form thioredoxin system with thioredoxin and NADPH. The activity of TrxR is similar with GR. TrxR could catalyzes GSSG reduct to GSH, which is the key enzyme in glutathione REDOX cycle.

TrxR catalyzes NADPH to reductDTNB form TNB and NADP+, TNB has a absorbance at 412 nm, but reduced glutathione  reacts  with  DTNB  to  form  TNB,  so  the  2-Vinylpyridine  in  this  kit  can  inhibit reduced glutathione in sample. The activity of TrxR can be calculated by detecting increase rate of TNB at

 

412 nm.

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