NAD Malic Enzyme(NAD-ME) Activity Assay Kit

Note: Take two or three different samples for prediction before test.

Detection equipment: Spectrophotometer

Cat No:AK0484

Size:50T/48S

Description:

MEwidely exists in microbe, culturing cells, animal and plant cytoplasm. Especially it has high activity in plant  tissue.  ME  catalyzesthe  reversible  reaction  of malic  acidoxidative  decarboxylation  to  produce pyruvic  acid  and  CO2with  the  reduction  of NAD(P)+,  which  is  the  crucial  enzyme  of malic  acid metabolism. The activity of ME is related with biosynthesis and anti-oxidation. In recent years, the determination of plant ME activity become a hot spot in antioxidant research. According coenzymes specificity   and   substrate   specificity,   ME   is   divided   into   NAD-ME(EC1. 1. 1.38)   and   NADP- ME(EC 1.1.1.40).

 

NADP-ME catalyzesthe reduction of NADP+ to NADPH,detect the increase rate of NADPH at 340nm.

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