NADP Malic Enzyme(NADP-ME) ActivityAssay Kit

Note: Take two or three different samples for prediction before test.

Detection equipment:Spectrophotometer/Microplate reader

Cat No:AK0485

Size:100T/96S

Description

MEwidely exist in microbe, culturing cells, animal and plant cytoplasm. Especially it has high activity in plant tissue. ME catalyzes the reversible reaction of malic acid oxidative decarboxylation to produce pyruvic  acid  and  CO2  with  the  reduction  of NAD(P)+,  which  is  the  crucial  enzyme  of malic  acid metabolism. The activity of ME is related with biosynthesis and anti-oxidation. In recent years, the determination of plant ME activity become a hot spot in antioxidant research. According the coenzyme specificity  and  substrate  specificity,  ME  is  divided  into  NAD-ME  (EC1. 1. 1.38)  and  NADP-ME (EC 1.1.1.40).

 

NADP-ME catalyzes the reduction of NADP+ to NADPH, detect the increase rate of NADPH at 340 nm.

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