Pyruvate Kinase  (PK)  Assay Kit

Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer

Catalog Number: AK0540

Size:50T/48S

Product Description

Pyruvate Kinase (PK, EC 2.7. 1.40) is widely exists in animals, plants, microorganisms and cultured cells. It could catalyze the final step of the glycolysis process. PK is one of the major rate-limiting enzymes in the glycolysis process and one of the key enzymes for ATP production. Therefore, the determination of PK activity is of great significance.

 

PK  catalyzes  the  generation  of  ATP  and  pyruvate  from  phosphoenolpyruvate  and  ADP.  Lactate dehydrogenase  further catalyzed NADH and pyruvate to generate lactic acid and NAD+ . The NADH degradation rate can measured at 340 nm to reflect the activity of PK.

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