Coenzyme Ⅰ NAD(H) Content Assay Kit

Note ：It is necessary to predict 2-3 large difference samples before the formal determination. Operation Equipment: Microplate reader/Spectrophotometer

Cat No: AK0559

Size: 100T/48S

Product Description：

Coenzyme I include both reduced and oxidized forms and plays a role in hydrogen transfer in biological oxidation.  Oxidized  coenzyme  I  also  called nicotinamide  adenine  dinucleotide  (NAD+) which  is  the coenzyme of dehydrogenase. It is an important role in glycolysis, gluconeogenesis, tricarboxylic acid cycle and   respiratory   chain.   Intermediate   product   will   transfer   hydrogen   to   NAD   make   it   become NADH(reduced coenzyme I). NADH acts as a carrier for hydrogen and synthesizes ATP by chemosmosis coupling in the respiratory chain. NADH has important physiological significance in the body. It is closely related to substance metabolism, energy metabolism, anti-cell aging, anti-oxidation and the occurrence of some diseases. A decrease in coenzyme I levels in the body can lead to cell damage or death.

 

Extract the sample of NAD+ and NADH with acidic and alkaline extract solution respectively, NADH reduces the oxidized Thiazolyl Blue Tetrazolium Blue (MTT) to form formazan by hydrogen transfer from PMS, formazan 

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