ATP Content Assay Kit

Note: It is necessary to predict 2-3 large difference samples before the formal determination. Operation Equipment: Spectrophotometer/Microplate Reader

Cat No: AK0561

Size: 100T/96S

Product Description:

ATP (adenosine 5'-triphosphate) is found broadly in animals, plants, microorganisms and cultured cells, which  is  described  as  the  energy  currency  in  all  living  systems.  Detecting  the  content  of ATP  and calculating the level of energy charge can reflect the state of energy metabolism.

Hexokinase (HK) catalyzes the synthesis of glucose and ATP into 6-phosphate glucose. 6-phosphate glucose dehydrogenase further catalyzes the dehydrogenation of glucose 6-phosphate and NADP to form NADPH. NADPH has a characteristic absorption peak at 340 nm, and the absorbance ratio of NADPH is in direct proportion to contents of ATP.

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