The ketone body is an intermediate acetoacetic acid (ACAC), β-hydroxycoherate (BOH), and acetone.The amount of acetone in the ketone body is quite small, and it is absorbed after the production.Acetoacetic acid and β-hydroxyobutic acid enter the hepatic tissue via a blood flow, which is oxidized, and more energy is provided with citric acid cycles to those tissue, such as bone, myocardium, and renal cortex.

Under pH 7.0 and 37 ° C, BOH was produced by hydroxymentation of hydroxyhydrate in beta-hydroxybutyrate dehydrogenase (HBDH), and NAD + was reduced to NadH; at pH 8.8 and 37 ° C.Under the conditions, the Acac was reduced by HBDH to 3-hydroxybutyrate or decarboxylates, and NADH was oxidized into NAD +, NADPH had a characteristic absorption peak at 340 nm wavelength, by detecting the light-absorbance value change at 340 nm wavelength, BOH can be calculatedAnd the content of the ACAC thus calculates the content of the ketone body of the Sbon.

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