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Product content:Extract: 50 mL × 1 bottle of liquid, 4 ° C preservation;
reagent one: Anhydrous ethanol 60 \u0026 nbsp; ml × 1 bottle, self-preparation, room temperature;Reagent 2: powder × 1 (0.6 mL \u0026 nbsp; EPT is placed in an 8 mL reagent bottle), 4 ° C. Add 6.08 \u0026 NBSP; ML reagent, an oscillate, and the incomplete reagent can be stored in -20 ° C for January, it is recommended to save; according to the required amount according to the requirements before the test 2: reagent one (V: V \u003d 4: 21 The proportion of 4: 21 is formulated into a working fluid, currently dispelled, using incomplete working fluids can be stored for one week at 4 ° C;
reagent 3: powder × 1, 10 mg vitamin C, 4 Save at ° C. 1 ml extract was added before, and dissolved in full oscillate; EtOAc (EtOAc)
Product Description:
DPPH free radicals a very stable nitrogen center free radical, S One of the important indicators of the anti-oxidation capacity, widely used in antioxidant food, health products and drugs.
DPPH free radical has single electrons, and the alcohol solution is purple, and there is strong absorption at 515 nm. When there is an antioxidant, the DPPH free radical is removed, and the color of the solution is shallow, and the absorbance of 515 nm is lowered, and the change in the absorbance in a certain range is proportional to the extent of free radical clearance. In this kit, the ability to reflect the S this clearance of DPPH free radicals by decreased absorbance.\u0026 nbsp; Agential Experimental Supplies:
Visit spectrophotometer, constant temperature water bath, 1 ml glass ratio Tools, desktop centrifuges, anhydrous ethanol, mortar / pulverizer, drying box, 30 ~ 50 mesh sieve and distilled water.
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