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Determination principle:PFP catalytic 6-phosphate fructose into 1,6-di phosphate jug, it is in aldolase and phosphosome isomerase The action was transformed into 3-phosphoric acid glyceryl, and then 3-phosphate dehydrogenase and NADH catalyzed 3-phosphouric acid, NAD and phosphoric acid, and the absorbance variation at 340 nm reflected the activity of PFP. Need instruments, consumables: balance, low temperature centrifuge, mortar, ultraviolet spectrometry / enzyme symbol, trace quantity-free cuvette / 96-well plate.
Determination significance:
Pyphosphate: fructose-6-phosphate-1-phosphate (PFP, EC2.7.1.90) is a cytoplase, widely found in plant tissue In the reversible conversion of catalytic fructose-6-phosphate and fructose-1,6-diphosphate, it plays an important role in photochariting carbon metabolism.
Scheme:
PFP catalytic 6-phosphate jug of 1,6-di phosphate jug, is converted to 3- The phosphorched aldehyde was then catalyzed from 3-phosphate dehydrogenase and NADH to form 3-phosphouric acid, NAD and phosphoric acid, and the absorbance variation at 340 nm reflected the activity of the PFP. Need instruments, consumables: balance, low temperature centrifuge, mortar, ultraviolet spectrometry / enzyme symbol, trace quantity-free cuvette / 96-well plate.
\u0026 nbsp;
Username | Quantity | bought time |
Li*** | 1 | 2024-08-05 |
Na*** | 2 | 2024-06-19 |
Pe*** | 1 | 2023-12-09 |
Za*** | 2 | 2023-12-01 |
Te*** | 1 | 2023-11-13 |
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