Measuring significance: Soil nitrous reductase is one of the key enzymes in denitrification, involved in the reduction reaction of nitrite to NO, and its activity reflects the biodegradation process.The conversion efficiency of nitrogen provides a certain basis for the study of nitrogen conversion regulations.

Measurement principle: Nitrite reductase can reduce NO2-reduction to NO, so that NO2-reduced NO2-reduction in the S product in the diazonation reaction, that is, the variation of the absorbance value at 540 nm can react in the soilActivity of nitrite reductase.

Need instruments, consumables: balance, mortar, visible spectrophotometer / enzyme standard, micro quad-British biopsy / 96-well plate, water bath, low temperature centrifuge.

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