Measurement Significance: THL (EC 3.2.1.28) is widely present in animals, plants, microorganisms and cultured cells.The main function of the seaweed sugar is that the biomerical decomposition trehalose is used to carry glucose and is directly supplied directly.

The principle of assay: The content of reducing sugars produced by THL catalyzed by THL catalyzed by 3.5-dinitropyrocyanic acid method.The reducing sugar and 3.5-dinitropylastocaricylic acid are used to generate brown red amino compounds, and the amount of reducing sugar in a range and the color depth of the reaction liquid is proportional to the high and low of THL activity.

Need instruments, consumables: visible spectrophotometer / enzyme label, water bath, adjustable pipette, trace quantine-based cuvette / 96-well plate, mortar, ice and distilled water.

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