Measurement Significance:

Glucose is not only the main substrate of cell energy metabolism, but also its metabolic intermediate product is an important substrate for biosynthesis.Plants can produce glucose by photosynthesis.For mammals, glucose is not only a unique energy of the brain nervous system, muscle, adipose tissue, but also closely related to the synthesis of reducing coenzyme, lactose and cream. Determination principle:

glucose oxidase catalyzes glucose oxidation into glucose acid, produces hydrogen peroxide; catalyzed hydroxide oxide oxidized 4-aminamine replanion coupling phenolThe colored compound is generated, and there is a feature absorption peak at 505 nm.

Required instruments, supplies: visible spectrophotometer / enzyme, water bath, adjustable pipette, trace quantum-based cuvette / 96-well plate, mortar and distilled water

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