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Measuring significance: chymotinase, also known as the pancreatic tractinase, is a proteolytic enzyme secreted by pancreas, which can quickly decompose denature protein.The function of the chymotridase is similar to trypsin, but has the advantages of strong decomposition ability, low toxicity and small reactions.Clinical chymosurinase is used for sputum dilute, which is effective for oculometers; it is also used for trauma or post-surgical wound healing, such as cataract removal.Measurement principle: Chimodide catalyzes ATEE hydrolysis, the product is characterized by tonifying light absorption at 237 nm; the diiminase activity is calculated by determining 237 NM light absorption increase rate.
Need instruments, supplies: desktop centrifuge, water bath, adjustable pipette, mortar, ultraviolet spectrometry / enzyme symbol, trace quantum-based cuvette / 96-well plate, ice and distilled water.
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