Measurement Significance: Multi-galacturonicase (EC3.2.1.15) is a cell wall binding protein that can catalytic pectin molecules α- (1, 4) -The cleavage of polypronic acid, participates in the degradation of pectin, causing the cell wall structure, resulting in fruit softening, ripening with fruit, falling off, pathogen defense, cell stretch development, and wooding, in plant disease resistanceIt has a high research value in the field of food storage.

Measurement principle: Multi-galacturonic acid enzyme acid generates galacturonic acid, has a reducing aldehyde group, reacting with DNS reagent, producing red-brown substance, with a characteristic absorption peak at 540 nm, and assay 540 nmThe variation of the absorbance value can be calculated to calculate the polylactonic acid enzyme activity.

Need instruments, consumables: balance, low temperature centrifuge, visible spectrophotometer / enzyme symbol, trace quantum-based cuvette / 96-well plate, constant temperature water bath.

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