Measurement Significance: xylanase (EC 3.2.1.8) is mainly produced by microorganisms, catalyzes hydrolysis of xylosans, also known as phenylganase or hemicellulase. , Can decompose the cell wall and β-glucan in the brewed or feed industry, reduce the viscosity of materials in the brewing, promote the release of effective substances, and reduce non-starch polysaccharides in the feed, promote the absorption and utilization of nutrients, and thus extensive Based on the brewing and feed industry, NEX is generally separated from microorganisms that are most suitable for growth pH.

Determination principle: NEX Catalyzes xylosaccharide into a reducing oligosaccharide and monosaccharide in a neutral environment, and has a developing reaction with 3,5-dinitrogenated salicylic acid under boiling water bath. At 540 nm, there is a characteristic absorption peak, the depth of the reaction liquid color is proportional to the amount of reduction sugar produced by the enzymatic hydrolysis, and the NEX viability can be calculated by measuring the rate of reaction of the reaction solution at 540 nm absorbance value.

Required instruments, supplies: balance, low temperature centrifuge, constant temperature water bath, visible spectrophotometer / enzyme symbol, trace quantum-based cuvette / 96-well plate and distilled water.

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