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Measurement Significance: β-Gal (EC 3.2.1.23) is widely present in animals, plants, microorganisms, and cultured cells, catalyzes β galactoside bonds in beta galactoside compounds.Hydrolysis, which also has the effect of turning galactoside.The β-Gal can not only release the energy stored energy, but also catalyze the hydrolyzate of polysaccharide, glycoprotein, and galactose and galactin and galactose., Release free galactose.Determination principle: β-gal decomposition pair-nitrobenzene-β-D-pyranium galactoside to-nitrophenol, the latter has the maximum absorption peak at 400 nm, by measuring the absorption valueRate to calculate β-GAL activity.
Need instruments, consumables: visible spectrophotometer / enzyme label, desktop centrifuge, water bath, adjustable pipette, trace quantum-based cuvette / 96-well plate, mortar, ice and distilled water.
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