Measurement Significance: The optimum pH of AI is 3 ~ 5.AI is divided into two types of soluble Ai (S-AI) and cell wall insoluble Ai (B-Ai).S-Ai mainly present in the cellular bubble or free space, the most suitable pH is 4.5 to 5.0 (acidic), by degrading the sucrose in the liquid, regulating the utilization of sucrose in the liquid and the accumulation of fruit lactose.

Measurement principle: Ni catalyzed sucrose decomposition to produce reducing sugar, further reacting with 3,5-dinitrogen-bicylic acid, generating brown red amino compound, characterized by characterizing light absorption in 510 nm, 510 nmThe absorption value is proportional to the amount of reducing sugar.The instrument required for Ni activity

is calculated by light absorption increase rate: visible spectrophotometer / enzyme label, desktop centrifuge, water bath, adjustable pipette, trace quantum-based cattle / 96Dental plate, mortar, ice and distilled water.

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