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Measurement Significance:FAS is a fatty acid synthesis of key enzymes, catalyzes acetyl co-enzyme A and properiloxidase A to produce long chain fatty acids.FAS is generally expressed in various tissue cells, which is rich in mammalian liver, kidney, brain, lung and breast and adipose tissue. As determined:
Fas catalyzed acetyl COA, malodyl COA and NADPH to produce length chain fatty acids and NADP +; NADPH has absorption peaks at 340 nm, and NADP + did not; decreased by determination of 340 nm light absorptionRate, calculate FAS activity.Instruments, consumables:
mortar, ice, desktop centrifuge, UV spectrophotometer / enzyme symbol, trace quantum-based cuvette / 96-well plate, adjustable pipetteGun and distilled water.
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