Measurement Significance: Blood phosphorus mainly refers to inorganic phosphorus insecius, exists in the form of inorganic phosphorus salt.The relationship between calcium and phosphorus in plasma is closely related, and the product ([Ca] × [P]) of the two is 30 to 40 when indicated by Mg / DL.When ([Ca] × [P]) \u0026 gt; 40, calcium and phosphorus are deposited in bone tissue in bone salts; if ([Ca] × [P]) \u0026 lt; 35 hinders the calcification of bone, even make boneSalt dissolution, affecting osteoblasts.The relative stability of blood calcium and phosphorus content is dependent on calcium, phosphorus absorption and excretion and calcification and two metabolism of decimination.The above equilibrium is adjusted by hormone such as vitamin D3, parathyroidin and calcitonin.

Determination principle: After removing organic phosphorus in serum, the inorganic phosphorus salt produces phosphomolybdic acid to molybdate and is reduced by ferrous sulfate, with light absorption at 660 nm; by determining 660 NM absorbance, Calculate the phosphorus content in the blood.

Instruments, consumables: centrifuge, adjustable pipette, visible spectrophotometer / enzyme symbol, trace quantum-based cuvette / 96-well plate, and distilled water.

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