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Measurement Significance:Several buttery mainly exists in the shell of shrimp, crab, insects, the outer casing of the shell and the organ of the mollus, such as the cartilage of squid),And in the cell wall of the fungi.The neutty enzyme (EC 3.2.1.14) can catalyze a tantin hydrolysis, with the role of resisting fungi infection, becoming a research hotspot of antifungal disease.
: Severall hydrolysis sects of N-acetamin glucose, further with red compounds to dimethylaminobenzaldehyde, at 585 nmThere is a feature absorption peak, and the increase rate of the absorbance value reflects the activity of a chitinase.
Required instruments, supplies:balance, water bath, centrifuge, visible spectrophotometer / enzyme symbol, trace quantum-based cuvette / 96-well plate, toluene (soil S productDedicated) and distilled water.
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