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Measurement Significance:Acetaldehyde dehydrogenase (EC 1.2.1.10) is one of aldehyde dehydrogenase, widely existing in various animals, plants andMicroorganisms.The main role is to oxidize the acetaldehyde into acetic acid, which plays a major role in alcohol metabolism.In humans and many other animals, mitochondrial acetaldehyde dehydrogenase transforms alcohol-like alcohols detrimental to organisms, so the acetaldehyde dehydrogenase is highly concerned in cell detoxification research; at the same time, acetaldehyde dehydrogenase is in molecular biology.And there is a wide range of research applications in terms of testing. Determination Principle: Under the conditions of coenzyme I, acetaldehyde dehydrogenase catalyzed by acetaldehyde and NAD +, which increased at 340 nm at 340 nm. Light at 340 nm.Value changes, the activity of the acetaldehyde dehydrogenase can be calculated.
Shentian experimental supplies and instruments: balance, centrifuge, constant temperature water bath, ultraviolet spectrophotometry, 1 mL quartz biopsy, distilled water.
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