Determination principle:

FBP catalyzes 1,6 diaphragin and water to generate 6 phosphate jug and inorganic phosphorus, adding phosphate glucose isomerase added in the reaction system The FBP activity can be calculated by catalyzing 6-phosphate dehydrogenase to form 6-phosphate gluconic acid and NADPH, 340 nm to determine the NADPH increase rate.

Instruments, consumables:

Spectrophotometer / enzyme symbol, constant temperature water bath, desktop centrifuge, adjustable pipette, trace quantum-based cuvette / 96-well plate, Drug, ice and distilled water. Measuring significance: Fruit-1,6 diphosphate is also known as fructose 1,6 diphosphate esterase, catalyzed 1,6 di phosphate jug and water to generate 6 phosphate jug and inorganic phosphorus, in sugar is born metabolism and photosynthetic The synthesis of sucrose plays a key role. Determination Principle:

FBP catalyzed 1,6 di-phosphate and water to generate 6 phosphate jellycetose and inorganic phosphorus, adding phosphate isomerase and 6-phosphate dehydrogenase added in the reaction system to catalyze 6 - FBP activity can be calculated from phosphate gluconic acid and NADPH, 340 nm. Required instruments, supplies: spectrophotometer / enzyme label, constant temperature water bath, desktop centrifuge, adjustable pipette, micro quarter-cuvette / 96-well plate, mortar, ice and distilled water.

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