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Measurement Significance: Fruit-1,6 diphosphatase is also known as fructose 1,6 diphosphate esterase, catalyzed 1,6 diphosphate jug and water to form6 phosphate jug and inorganic phosphorus, a key role in the synthesis of sugar in the synthesis of sugar. As determined:
FBP catalyzed 1,6 di phosphate jug and water to generate 6 phosphate jug and inorganic phosphorus, adding phosphate isomerase and 6-phosphate dehydrogenase added in the reaction systemFBP activity can be calculated to form 6-phosphate gluconic acid and NADPH, 340 nm to determine the NADPH increase rate. Required instruments and supplies: Spectrophotometer, constant temperature water bath, desktop centrifuge, adjustable pipette, 1 mL quartz biopsy, mortar, ice and distilled water.
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