Measurement Significance: \u0026 NBSP; amylase is responsible for hydrolyzing starch, mainly including alpha-amylase and β-amylase.β-amylase (EC 3.2.1.2) can randomly act on α-1,4-glycosidic bonds in starch, forming reducing sugars such as glucose, maltose, maltosaccharide, dextrin.

Determination Principle:

Fe2 + and Phiorerazine Reacts to form a purple compound, and there is a characteristic absorption peak at 562 nm.Under alkaline conditions, serum transferrin can be bonded to Fe3 +, and the remaining unbound Fe3 + can be reduced to Fe2 +, at which point absorbance A1 is positively correlated with the number of FE3 +; after acidification, the ferrin binds FE3 + release, and furtherIt is restored to Fe2 + \u0026 nbsp; at this time, the absorbance A2 is positively correlated with the total FE3 + quantity.The A2 reduction A42 is proportional to the TiBC concentration. Required instruments, consumables:

visible spectrophotometer / enzyme label, constant temperature water bath, centrifuge, adjustable pipette, trace quantum-based cuvette / 96-well plate,Drug and distilled water.

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