Measurement Significance:

α-KGDH (EC1.2.4.2) is widely present in mitochondria of animals, plant microorganisms, and cultured cells, and is tricarboxylic acid cycles.Control the key enzyme, catalyze alpha-ketorate oxidation decarboxylation to form succinyl concentrate A. Measurement principle: α-KGDH Catalyzed α-ketoxic acid, NAD + and coenzyme A to form succinyl concentrate A, carbon dioxide and NADH, NADH with characteristic absorption peaks at 340 nm, with NADH-generating rateIndicates α-KGDH activity.
Instruments and reagents needed in the test: Ultraviolet spectrophotometer, water bath, desktop centrifuge, adjustable pipette, 1ml quartz biopsy, mortar, ice and distilled water.

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