Determination ofSignificance: α-KGDH (EC 1.2.4.2)exists widely inanimals, plants,microorganisms andculturedcellsmitochondria,arecitric acid cycleregulationone of the keyenzyme that catalyzes theoxidative decarboxylation ofα-ketoglutarategeneratesuccinyl-CoAA.

Measurementprinciple: α-KGDHcatalyticα-ketoglutarate, NAD +to generatesuccinyl-coenzyme Aandcoenzyme A,carbon dioxide andNADH, NADHat340 nmcharacteristic absorptionpeaks,inNADHrepresents thegeneration rateofα-KGDHactivity.

needs, consumables:UV spectrophotometer/Reader,water bath,desktop centrifuge,adjustablepipettes,microquartzcuvette/96,mortar,iceand distilled water.

Cartpieces

• 
• 4

  4Delete

• 
• 4

  4Delete

• 
• A

  ADelete

• 
• �

  �Delete

• 
• +

  +Delete

• 
• 1

  1Delete

• Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 3

  3Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• �

  �Delete

• 
• �

  �Delete

• 
• <

  <Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• 3

  3Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• �

  �Delete

• 
• 4

  4Delete

• 
• A

  ADelete

Totalgoods,subtotals:￥Checkout