Measuring significance:

Reducing sugar is widely present in animals, plants, microorganisms and cultured cells.Reducing sugar in the plant mainly includes glucose, fructose and maltose, etc., is the most common monosaccharide and double sugar, where glucose and fructose are not only the main substrate of breathing, but also the substrate of sucrose, starch and cellulose. Measurement Principle: Heating to promote the 3,5-dinitrogenated salicylic acid solution in the alkaline solution to form a brown red amino compound in the reducing sugar, and there is a characteristic absorption peak at 540 nm; in a certain concentration rangeInside, the reducing sugar content is linearly related to 540 nM absorbance, and the amount of reducing sugar in S is obtained according to standard curves.
The instrument and reagent required in the test: Visit spectrophotometer, water bath, adjustable pipette, 1ml glass cuvette, mortar, distilled water.

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