Measurement Significance: Pyruvate Carboxylase, PC, EC 6.4.1.1) is widely present in mitochondria, catalyzed pyruvate, ATP, and CO2 in mitochondria, catalyzed pyruvate, ATP, CO2And the aqueous hydroformate, ADP and Pi are the first speed limit enzyme in the syndrome of the sugar, which plays an important role in ensuring the dynamic balance of blood sugar.

Measurement principle: PC catalyzed pyruvic acid, ATP, CO2 and water into oxfloy acetic acid, ADP and PI, maline dehydrogenase further catalytic oxamic acid and NADH to form malic acid and NAD +, measured at 340 nmNADH oxidation rate can reflect the instrument required for PC activity

, supplies: spectrophotometer / enzyme label, desktop centrifuge, adjustable pipette, trace quantum-based cuvette / 96-well plate, researchBottling, ice and distilled water.

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