Measurement Significance: Pyruvate Carboxylase, PC, EC 6.4.1.1) is widely present in mitochondria, catalysts of animals, mold and yeastPyruvic acid, ATP, CO2 and aqueous formation of oxamic acid, ADP and Pi are the first speed limit enzyme in the syndrome of the sugar, which plays an important role in ensuring the dynamic balance of blood sugar. Determination Principle:
PC catalyzed pyruvic acid, ATP, CO2 and aqueous generated oxyl acetic acid, ADP and PI, malic dehydrogenase further catalytic oxamic acid and NADH to form malic acid and NAD +, inThe NADH oxidation rate was determined at 340 nm, and the PC activity can be reflected. Required instruments and supplies: Spectrophotometer, desktop centrifuge, adjustable pipette, 1 mL quartz biopsy, mortar, ice and distilled water.

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