Determination Significance: Chitin mainly exists in the shells of crustaceans such as shrimp, crab, insects and mollusk organs (such as squid cartilage), as well as the cell wall of fungi, and chitin enzyme (EC 3.2.1.14) can catalyze the hydrolysis of chitin, has the role of resistance to fungal infection, has become a research focus of anti-fungal diseases.

Determination principle: chitinase hydrolyzes chitin to produce n-acetyl glucosamine, and further produces red compound with p-dimethylaminobenzaldehyde. The characteristic absorption peak at 585nm is observed. The increasing rate of absorption value reflects the activity of chitinase.

Required instruments and consumables: balance, water bath, centrifuge, oscillator, visible spectrophotometer/microplate reader, quartz cuvette / 96-well plate, toluene, distilled water.

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