NR (EC 1.7.1.3) widely exists in plants, and is the key enzyme and inducing enzyme for converting nitrate nitrogen to ammonia nitrogen, which has an impact on crop yield and quality. Nitrate could be reduced to nitrite catalyzed by NR, with NO3ˉ +NADH+H+→NO2ˉ+NAD++H2O. Under acidic conditions, the NO2ˉ could participate in diazotization reaction and produce purplish red compound. The purplish red compound had an absorption peak at 540 nm, and the change of the absorbance value at 540 nm could represent the enzyme activity.

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