Product Description: Caspase is a family of proteases involved in the process of apoptosis, including more than 10 members. Caspase-2, also known as Ich-1 or Nedd-2, is activated in the process of apoptosis signal transduction. There are two different splicing variants of caspase-2 mRNA. The protein translated by the full-length mRNA can promote apoptosis, while the short protein product can inhibit apoptosis. Caspase 2 can be activated by caspase- 1, caspase-3 and granzyme B. The caspase-2 colorimetric assay is based on the hydrolysis of the peptide substrate Nacetyl-Val-Asp-Val-Ala-Asp-pNA (Ac-VDVAD-pNA) by caspase-2, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline has a high absorbance at 405 nm. The activity of Caspase can be calculated by detecting pNA. This kit is suitable for mammalian tissue and cell.

Reagents and Equipment Required but Not Provided: Spectrophotometer/Microplate reader, 100μL cuvette/ 96-well plate, centrifuge, water bath / incubator, adjustable pipette, mortar / homogenizer, ice and distilled water 

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