Product Description: Caspase is a family of proteases involved in the process of apoptosis, including more than 10 members. Caspase-1 is the only caspase that can cleave IL-1b and IL-18 precursors to produce active cytokines. Caspase-1 regulates apoptosis by cleaving BCL XL, and regulates cytokine mediated immune-inflammatory response by cleaving cytokine precursors. The caspase-1 colorimetric assay is based on the hydrolysis of the peptide substrate Tyr-Val-Ala-Asp-p-nitroanilide (YVAD-pNA) by caspase-1, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline has a high absorbance at 405 nm. The activity of Caspase can be calculated by detecting pNA. This kit is suitable for mammalian tissue and cell.

Reagents and Equipment Required but Not Provided: Spectrophotometer/Microplate reader, 100μL cuvette/ 96-well plate, centrifuge, water bath / incubator, adjustable pipette, mortar / homogenizer, ice and distilled water

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