作为土壤中一类重要的化合物，氨基酸占土壤中全氮的15%-60%，是土壤氮素循环和植物养分供给过程中重要的“库”和“源”，可通过矿化作用满足作物氮素营养的需求，也可以被植物直接吸收利用。另外，氨基酸也是土壤微生物重要的氮源，对于土壤微生物群落结构、数量和活性均有直接影响。因此，研究土壤中氨基酸含量变化规律，对研究土壤氮素循环，及其对植物生理生态变化的影响均有重要意义。

氨基酸的α-氨基可与水合茚三酮反应在570nm有特征吸收峰；通过测定570nm吸光度，来计算氨基酸含量。

As a kind of important compounds in soil, amino acids account for 15%-60% of total nitrogen in soil, and are important "reservoir" and "source" in the process of soil nitrogen cycling and plant nutrient supply. They can meet the requirements of crop nitrogen nutrition through mineralization, and can also be directly absorbed and utilized by plants. In addition, amino acids are also important nitrogen sources for soil microorganisms, which have a direct impact on soil microbial community structure, number and activity. Therefore, it is of great significance to study the variation of amino acid content in soil for the study of soil nitrogen cycling and its effect on plant physiological and ecological changes.

The α -amino acid of amino acid can react with hydrated ninhydrin and have characteristic absorption peak at 570nm. The amino acid content was calculated by measuring the absorbance at 570nm.

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