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Purpose
Our Canine Soluble Cluster of differentiation 335,sCD335 ELISA Kit is to assay sCD335 levels in Canine serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to sCD335. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for sCD335 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain sCD335 and HRP conjugated sCD335 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of sCD335. You can calculate the concentration of sCD335 in the samples by comparing the OD of the samples to the standard curve.
Username | Quantity | bought time |
Iv*** | 1 | 2021-09-19 |
Ol*** | 2 | 2020-12-21 |
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