Chinese Name	胶原酶Ⅰ
ALIAS	Paracetic peptide A
Appearance	Brown crystalline powder
Source	Collagenase from CLOSTRIDIUM Histolyticum
Solubility	5 mg/mlWater

 

 

Collagenase Ⅰ is used for separation of tissue cells in epithelial, lung and adrenal glandular tissue

Collagin enzymes are derived from soluble tissue stunaches

collagense Ⅰ can be possible Use TESCA BUFFER (50 mm Tes, 0.36 mm calcium chloride, pH 7.4, 37 ° C) to make 1-2 mg/ml. You can also use calcium -magnesium -containing PBS, calcium magnesium Hanks, or serum -free training. After the matching is finished, avoid repeated freezing.

1 collagen digestive unit (CDU): under the conditions of pH 7.4, 37 ° C, and calcium ions, the polypeptide released from the collagen every 5 hours is equivalent to the amount of ketone coloring 1 microline. It is an active unit.
1FalgPa unit: Under the conditions of PH 7.5, 25 ° C and calcium ions, a Falgpa hydrolyzed unit can hydrolyze 1.0 μmol acryl-Leu-GLY-PRO-ALA.
Collase Ⅰ is a rough product, which is used for separation of tissue cells in epithelial, lung, and adrenal glandular gland. The appearance is black brown powder. Molecular weight is 68,000 to 125,000, and the most suitable pH is 6.3-8.8. The collagen enzyme can be specific to bind the peptide bonds between the neutral amino acids (X) and glycine in the-R-PRO-8-X-GLY-PRO-R-sequence. This rough product is a mixed enzyme secreted by the soluble tissue. Mainly collagense, neutral protease and dehioxylinase. Among them, dermal proteases are oxidized and most lively enzymes. The separation of tissue must rely on collagenase and neutral protease in common.

Activant: CA2+, the most suitable concentration is 5 mm.

Inhibitors: EGTA, reducing glutathione, β-cymbal ethanol, sodium cymbal ethyl, 8-hydroxylinoline, 2,2 \u0026#39; -lianyridine, etc.

Different types: Different types of collagen are their natural substrates, and some synthetic polypeptides can also be used as their substrates, such as: N-CBZ-GLY-Pro-Gly-PRO-ALA (KM (KM (KM (KM (KM (KM (KM \u003d 0.71 mm), N-CBZ-GLY-PRO-Leu-Leu-GLY-PRO, N- (3- (2-Furyl) acryloyl)-leu-gly-pro-ala (FALGPA), 4-PHENYLAZO BENZYLORBORBORBONYL-PRO- Leu-GLY-PRO-D-RG, N-2,4-Dinitrophenyl-Pro-GLN-GLY-ALA-GLY-GLN-D-RG. In addition, N-Succinyl-Gly-Pro-Leu-GlyPro7-AMIDO-4-METHYLCOUmarin is a substrate for peptidases of collains.N- (2,4-Dinitropheneyl) -Proleu-Gly-Leu-TRP-ALA-ALA-ALA-ALG amino acid compound is a substrate for vertebrate colloidase.

\ Remarks: Product information may be optimized and upgraded.Please refer to the actual label information.

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