Chinese Name	胶原酶III
ALIAS	Paracetic peptide A
Appearance	Brown frozen dried powder
Solubility	5 mg/mlWater

 

The connection part of the digestive tissue makes it a single cell, which is used for mammalian tissue cells. -10 FALGPA units/mg solid, ≥250 CDU/mg solid (CDU \u003d collagen digestion units), lyophilized powder
Biochem/physiol Actions
Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.
Crude collagenases are mixtures of enzymes (mostly proteases) secreted by C. histolyticum. Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different Proteases Ranging in Molecular Weight from 68-130 kda.
Packaging
Product Sold on the Basis of Collagestion Units (CDU).
Preparation Note
Collagenase may be dissolved in TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4 at 37 °C) at 0.05-0.1 mg/mL concentration.
Unit Definition
One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly -Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.
Application
Collagenase from Clostridium histolyticum may be used for the isolation of cells from many types of animal tissue. It may be used for the disaggregation of human tumor, mouse kidney, Human adult/fetal brain, lung and many other tissues. It may also be used in liver50 mm TES, 0.36 mm Calcium Chloride, PH 7.4): Soluble0.05-0.1 mg/ml at 37 ° C
Foreign action clostribain, may contain trace amount neutral process
storageC




Remarks:

Product information may be optimized and upgraded.Please refer to the actual label information.<1 unit/mg solid

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