Storage	-80 ° C, dry ice transportation
Validity	2 Years
Titer	5U/ul
ALT Name	ULP

 

Sumo protease, also known as ULP, is a cysteine \u200b\u200bprotease with high activity. It can identify the three -level structure of Sumo protein rather than the amino acid sequence. Therefore Cut it on the fusion protein. SUMO protease can maintain higher activity in a wide range of reaction environment systems, such as temperature (4-30 ° C), pH (5.5-9.5), etc., Sumo protease has a multi-clustered HIS label, which is convenient for fusion protein cutting after cutting of protein cutting after cutting protein cutting. The affinity layer removes the protease.

 

Save conditions:

Sumo protease -80 ° C for a long time, which can be stored for 2 Yearss; Try to melt. 10 × Sumo Protease Buffer can be stored at -20 ° C.

Enzyme activity definition:

In 1 × Sumo Protease Buffer (50 mm tris-hCl, 1 mm dtt, pH 8.0) The amount of enzymes required for 2 μg substrate is defined as an active unit.

How to use:

1. 10 × Sumo Protease Buffer: 500mm Tris-HCl, 10mm DTT, PH 8.0.

2. The proportion of protein in the purpose of sumo protease to enzyme cutting: 1: 100.

3. Enzyme cutting system:

Fusion protein 1000 μg

10 × Sumo Protease buffer 20 μl

[

[ [ [

[

[ [ 123] Sumo protease

2μL

DDH2O fixed capacity to

1000 μl The target protein studied by myself is explored. The following enzyme cutting analysis pictures are available for reference. 5. After the enzyme is cut, a small amount of samples can be taken for SDS-PAGE analysis. If you want to remove the Sumo protease in the system, you can use the HIS tag to purify resin affinity.

Remarks: Product information may be optimized and upgraded. Please refer to the actual label information.

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