Storage	-80 ° C, dry ice transportation
Validity	2 Years
Titer	5U/ul
ALT Name	RTEV
Appearance	Colorless transparent liquid

 

RTEV protease (reorganization) is a restructuring protease after genetic engineering transformation, the enzyme-specific sexual sex Glu-Asn-Leu-Tyr-PHE-GLN-GLY sequence. Compared with proteases such as intestinal kinase U (EK) U (EK), THROMBIN, Factorxa, Sumo, it has the dual characteristics of high activity and high -specificity. Because of high shear activity and specificity, RTEV protease has become a fusion protein. After expression, remove the preferred protease of fusion TAG. The enzyme is purified by 6 × HIS tags (including the arcered acid tags), and the purity is 99 %. Purpose of purifying the purpose of protein.

 

Table

1 . At different temperatures RTEV Enzyme cutting activity

The response time

16 ° C 21 ° C 1 h 3] 58 70 2 h 58 ] 99 3.5h Pure: \u0026 gt; 95%, as determined by sds-page SDS-PAGE:

不同温度下剪切活性(%)	[123 ] 4 ° C
		30 ° C		34 [123 ]
56	]			80
78	71	99	99
84	99	99	123] 99	123]
Common factor affecting natural TEV enzyme activity: 1) PMSF and AEBSF (1 mm), TLCK (1 mm), Bestatin (1 mg/ml), Pestatin A ( 1 mm), EDTA (1 mm), and E-64 (3 mg/ml). The above protease inhibitors have no effect on the activity of RTEV; 2) Zn ion (\u003e 5 mm) inhibit the activity of RTEV; 3) and and and and and. The reagent of cysteine \u200b\u200breaction is also a potential inhibitor of RTEV.	expression method: A DNA Sequence Encoding the Tev Protease Terminus, with a Ser 2256 Asn Mutation species: Human Expressing host: e.coli qc testing	: \u0026 lt; 0 1.0 EU Per 1 μg Cytokine as determined by the Lal Method. Stability: Samples are stable for up to twelve months from date of receipt Met 1 Molecular weight: The Recombinant Human TEV Protease Consist of 251 Amino Acids and Has A Predict Molecular Mass of 28.6 KDa. As estimated in SDS-PAGE Under Reducing Conditions. Caps: SUPPLIED As a 0.2 μm Filtered Solution of 50mm Tris, 10%Sucrose, 5mm DTT PH8.0	usagge g Storage method: Store It Under Sterile Conditions at -70 ℃. It is recoming that the proverin be aliquoted for optional storage and usage.123] Note:	Product information may be optimized and upgraded.Please refer to the actual label information.

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