Product Name	Goat Anti-Rabbit IgG H&L / Biotin
Chinese Name	生物素标记的羊抗兔IgG H&L
Alias	Goat Anti-Rabbit IgG H&L (Biotin); Immunoglobulin G;
	Specific References  (21)     |     SL0295G-Bio has been referenced in 21 publications. [IF=7.561] Min Yang. et al. The Role of Renal Macrophage, AIM, and TGF-β1 Expression in Renal Fibrosis Progression in IgAN Patients. Front Immunol. 2021; 12: 646650  IF,IHC ;  Human.   PubMed:34194427 [IF=6.551] Nuanfei Zhu. et al. Dual amplified ratiometric fluorescence ELISA based on G-quadruplex/hemin DNAzyme using tetrahedral DNA nanostructure as scaffold for ultrasensitive detection of dibutyl phthalate in aquatic system. Sci Total Environ. 2021 Aug;784:147212   PubMed:33905933 [IF=5.81] Wei W. et al. Apigenin, a Single Active Component of Herbal Extract, Alleviates Xerostomia via ERα-Mediated Upregulation of AQP5 Activation.. Front Pharmacol. 2022 Feb;13:818116-818116  IHC ;  Mouse.   PubMed:35264956 [IF=5.395] Yuanchao Zhu. et al. Biomimetic Porous Magnesium Alloy Scaffolds Promote the Repair of Osteoporotic Bone Defects in Rats through Activating the Wnt/β-Catenin Signaling Pathway. ACS BIOMATER-SCI ENG. 2023;XXXX(XXX):XXX-XXX  IHC ;  Rat.   PubMed:37200162 [IF=5.227] Junwei Shi. et al. Electrochemiluminescence Imaging of Membrane Carcinoembryonic Antigen at Single Tissue Sections. ANALYST. 2023 Apr;:  IF ;  Human.   PubMed:37191134 [IF=5.177] Rong Cong. et al. The PVT1/miR-612/CENP-H/CDK1 axis promotes malignant progression of advanced endometrial cancer. Am J Cancer Res. 2021; 11(4): 1480–1502  IHC ;  Human.   PubMed:33948369 [IF=3.996] Chen, Qiliang. et al. Exploration of a Brain-Liver-Communication-Related Mechanism Involved in the Experimental Perimenopausal Depression Rat Model using Chaihu-Shugan-San. Neurochem Res. 2022 Feb;:1-15  IHC ;  Rat.   PubMed:10.1007/s11064-022-03534-y [IF=3.848] Chen Luyao. et al. Epicatechin gallate prevents the de novo synthesis of fatty acid and the migration of prostate cancer cells. Acta Bioch Bioph Sin. 2021 Oct;:  IHC ;  Mouse.   PubMed:34718375 [IF=3.297] Dan Zhang. et al. Screening and identification of a CD44v6 specific peptide using improved phage display for gastric cancer targeting. Ann Transl Med. 2020 Nov; 8(21): 1442  IF,IHC ;  Human.   PubMed:33313187 [IF=3.267] Asako Takahashi. et al. Erucic Acid-Rich Yellow Mustard Oil Improves Insulin Resistance in KK-Ay Mice. Molecules. 2021 Jan;26(3):546  IHC ;  Mouse.   PubMed:33494317 [IF=3.171] Jian-Zhu Bo. et al. D-serine reduces memory impairment and neuronal damage induced by chronic lead exposure. Neural Regen Res. 2021 May;16(5):836  IHC ;  Rat.   PubMed:33229717 [IF=3.145] Zheng Hua-chuan. et al. The potential oncogenic effect of tissue-specific expression of JC polyoma T antigen in digestive epithelial cells. TRANSGENIC RES. 2023 May;:1-15  WB ;  Mouse.   PubMed:37247123 [IF=3.098] Lu Xue. et al. A novel peptide relieves endothelial cell dysfunction in preeclampsia by regulating the PI3K/mTOR/HIF1α pathway. Int J Mol Med. 2021 Jan;47(1):276-288  IHC ;  Rat.   PubMed:33236147 [IF=2.923] Junyuan Ma. et al. Effects of melatonin on development and hormone secretion of sheep theca cells in vitro. THERIOGENOLOGY. 2023 Mar;198:172  IHC ;  Sheep.   PubMed:36592515 [IF=2.767] Dezhuan Da. et al. Glutamate-cysteine ligase catalytic and its modifier function as novel immunotargets in gastric adenocarcinoma. Asian J Surg. 2022 Mar;:  IHC ;  Other.   PubMed:35241341 [IF=2.65] Zaki Asmaa Hussein. et al. Medicinal Mushroom Leucocalocybe mongolica Imai Extracts Improve Mammary Gland Differentiation in Lactating Rats via Regulating Protein Expression. EVID-BASED COMPL ALT. 2022;2022:5762847  IHC ;  Rat.   PubMed:35761899 [IF=2.005] Yongfu La. et al. Different expression of LHR, PRLR, GH and IGF1 during testicular development of yak. 2021 Nov 09  IHC ;  Yak.   PubMed:34752661 [IF=1.858] Xingyu Min. et al. Analysis of PPP1R11 expression in granulosa cells during developmental follicles of yak and its effects on cell function. REPROD DOMEST ANIM. 2022 Oct;:  IHC ;  Yak.   PubMed:36178063 [IF=1.601] Songtao Zhang. et al. CMTM6 and PD-1/PD-L1 overexpression are associated with the clinical characteristics of malignancy in oral squamous cell carcinoma. Or Surg Or Med Or Pa. 2021 Mar;:  IHC ;  Human.   PubMed:10.1016/j.oooo.2021.02.019 [IF=1.41] Yi X et al. Expression of Keap1 and Nrf2 in diffuse large B‑cell lymphoma and its clinical significance.Exp Ther Med. 2018 Aug;16(2):573-578.  IHC-P ;  Rabbit.   PubMed:30112024 [IF=0] Yunsheng Wang. et al. A rare case of giant panda cancer: Pancreatic ductal adenocarcinoma. Animal Models and Experimental Medicine. 2022 Nov;:  IHC ;  Panda.   PubMed:36369766
Immunogen Species	Goat
Clonality	Polyclonal
React Species	Rabbit,
Applications	IHC-P=1:200  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Form	Liquid
Concentration	2.0 mg/ml
immunogen	Native rabbit IgG
Lsotype	IgG
Purification	affinity purified by Protein G, nonspecific adsorbed
Buffer Solution	10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
Storage	Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Product Detail	Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Product Picture	Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MBP) Polyclonal Antibody, Unconjugated (SL24827R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:500) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MBP) Polyclonal Antibody, Unconjugated (SL24827R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:500) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated (SL3796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:2000) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated (SL3796R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:2000) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:500) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:500) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:500) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:500) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:500) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:500) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:500) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated (SL0349R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:500) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (RAT brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (SL0199R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:1000) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (SL0199R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:1000) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MBP) Polyclonal Antibody, Unconjugated (SL24827R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:1000) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MBP) Polyclonal Antibody, Unconjugated (SL24827R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:1000) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (SL0199R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:2000) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (SL0199R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023 SL0295G-Bio 1:500) instructionsand DAB staining.

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