Product Name	Goat Anti-Rabbit IgG H&L / AF555
Chinese Name	Alexa Fluor 555标记的羊抗兔IgG H&L
Alias	Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555); Immunoglobulin G;
	Specific References  (32)     |     SL0295G-AF555 has been referenced in 32 publications. [IF=30.849] Yao Li. et al. Rapid surface display of mRNA antigens by bacteria-derived outer membrane vesicles for a personalized tumor vaccine. 2022 Mar 22  IF ;  Mouse.   PubMed:35315546 [IF=19.227] Xin Zhou. et al. ACSL4 promotes microglia-mediated neuroinflammation by regulating lipid metabolism and VGLL4 expression. BRAIN BEHAV IMMUN. 2023 Mar;109:331  IF ;  Rabbit.   PubMed:36791893 [IF=11.15] Zhan Yizhi. et al. NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinoma. BMC MED. 2022 Dec;20(1):1-19  IF ;  Human.   PubMed:36258210 [IF=8.079] Yuan, Yu. et al. Adipose-derived stromal/stem cells are verified to be potential seed candidates for bio-root regeneration in three-dimensional culture. STEM CELL RES THER. 2022 Dec;13(1):1-23  IF ;  Human.   PubMed:35659736 [IF=6.633] Yu Cui. et al. ACSL4 exacerbates ischemic stroke by promoting ferroptosis-induced brain injury and neuroinflammation. Brain Behav Immun. 2021 Jan;:  IF,IHC ;  Mouse.   PubMed:33444733 [IF=6.575] Qingqing Liu. et al. A Synbiotic Ameliorates Con A-Induced Autoimmune Hepatitis in Mice Through Modulation of Gut Microbiota And Immune Imbalance. MOL NUTR FOOD RES. 2023 Jan;:2200428  IF,IHC ;  Mouse.   PubMed:36708241 [IF=6.208] Qiyang Yao. et al. Oxidative Stress as a Contributor to Insulin Resistance in the Skeletal Muscles of Mice with Polycystic Ovary Syndrome. INT J MOL SCI. 2022 Jan;23(19):11384  IF ;  Mouse.   PubMed:36232686 [IF=5.722] Shi, Yanlong. et al. NCAPG facilitates colorectal cancer cell proliferation, migration, invasion and epithelial–mesenchymal transition by activating the Wnt/β-catenin signaling pathway. Cancer Cell Int. 2022 Dec;22(1):1-13  IF ;  Human.   PubMed:35292013 [IF=5.378] Tan et al. Upregulation of DACT2 suppresses proliferation and enhances apoptosis of glioma cell via inactivation of YAP signaling pathway. (2017) Cell.Death.Di. 8:e2981  FCM ;  Rabbit.   PubMed:28796248 [IF=5.047] Du YQ et al. A study of polyethylene glycol backfilling for enhancing target recognition using QCM-D and DPI.(2018) Journal of Materials Chemistry B.  CLSM ;  Rabbit.   PubMed:10.1039/c8tb01526k [IF=5.039] Luo P et al. IL‐37b alleviates inflammation in the temporomandibular joint cartilage via IL‐1R8 pathway. Cell Prolif. 2019 Sep 27:e12692.  ICF ;  Rabbit.   PubMed:31560411 [IF=4.967] Weijie Jiao. et al. Construction and Evaluation of Small-Diameter Bioartificial Arteries Based on a Combined-Mold Technology. POLYMERS-BASEL. 2022 Jan;14(15):3089  IF ;  Rat.   PubMed:35956602 [IF=4.966] Fan Li. et al. PHD3 mediates denervation skeletal muscle atrophy through Nf‐κB signal pathway. Faseb J. 2021 Apr;35(4):e21444  IF ;  Mouse.   PubMed:33749901 [IF=4.925] Yuan P et al. The nanomaterial-induced bystander effects reprogrammed macrophage immune function and metabolic profile. Nanotoxicology . 2020 Oct;14(8):1137-1155.  ICF ;  Rabbit.   PubMed:32916084 [IF=4.925]  Peng Yuan . et al. The nanomaterial-induced bystander effects reprogrammed macrophage immune function and metabolic profile. Nanotoxicology. 2020;14(8):1137-1155  IF ;  Human.   PubMed:32916084 [IF=4.329] Zhiyuan Zhao. et al. Design and Fabrication of Nanofibrous Dura Mater with Antifibrosis and Neuroprotection Effects on SH-SY5Y Cells. POLYMERS-BASEL. 2022 Jan;14(9):1882  IF ;  Human.   PubMed:35567051 [IF=4.254] Gao L et al. Restoration of E-cadherin by PPBICA protects against cisplatin-induced acute kidney injury by attenuating inflammation and programmed cell death. Lab Invest. 2018 Jul;98(7):911-923.  IF ;  Rabbit.   PubMed:29581579 [IF=4.145] Yu Sun. et al. Colorectal cancer-derived extracellular vesicles containing HSP70 enhance macrophage phagocytosis by up-regulating MARCO expression. EXP CELL RES. 2023 Mar;:113565  ICC ;  Mouse.   PubMed:36958650 [IF=3.292] Jun Yan. et al. Toxic effects of the food additives titanium dioxide and silica on the murine intestinal tract: Mechanisms related to intestinal barrier dysfunction involved by gut microbiota. Environ Toxicol Phar. 2020 Nov;80:103485  IF ;  Mouse.   PubMed:32891757 [IF=3.231] Jie Su. et al. Dosage Compensation of the X Chromosome during Sheep Testis Development Revealed by Single-Cell RNA Sequencing. ANIMALS. 2022 Jan;12(17):2169  IF ;  Sheep.   PubMed:36077890 [IF=3.169] Hak Myong Choe. et al. Altered fibrinogen level and fibrin clot structure in myostatin homozygous mutant pig. 2022 Mar 13  IF ;  Piglets.   PubMed:35285059 [IF=2.923] Pengyan Song. et al. miR-200b/MYBL2/CDK1 suppresses proliferation and induces senescence through cell cycle arrest in ovine granulosa cells. THERIOGENOLOGY. 2023 Sep;207:19  ICC ;  Sheep.   PubMed:37257219 [IF=2.85] Li, Hongyan, et al. "Comparative proteomics reveals the underlying toxicological mechanism of low sperm motility induced by iron ion radiation in mice." Reproductive Toxicology (2016).  IF(ICC) ;  Rabbit.   PubMed:27460355 [IF=2.68] Zhu Shumin. et al. High-fat diet and alcohol induced-mice could cause colonic injury through molecular mechanisms of endogenous toxins. TOXICOL RES-UK. 2022 Jul;:  IF ;  Human.   PubMed:10.1093/toxres/tfac025 [IF=2.68] Li, Hongyan, et al. "Comparative analysis of the serum proteome for biomarker discovery to reveal hepatotoxicity induced by iron ion radiation in mice." Life Sciences 167 (2016): 57-66.  IF(IHC-P) ;  Rabbit.   PubMed:27815023 [IF=2.554] Xin-Yue Liu. et al. Positive growth of smooth muscle in uterine horns of myostatin homozygous mutant gilt. RES VET SCI. 2022 Aug;:  IF ;  Pig.   PubMed:36027840 [IF=2.447] Bing Han. et al. Inhibition of autophagy promotes human RSV NS1‑induced inflammation and apoptosis in vitro. Exp Ther Med. 2021 Oct;22(4):1-9  IF ;  Human.   PubMed:10.3892/etm.2021.10488 [IF=2.1] Bing Li. et al. miR‑449a‑5p suppresses CDK6 expression to inhibit cardiomyocyte proliferation. Mol Med Rep. 2021 Jan;23(1):1-1  IF ;  Mouse.   PubMed:33179102 [IF=1.86] Zhang, Yaning, et al. "Identification of Mycoplasma suis MSG1 interaction proteins on porcine erythrocytes." (2014). Archives of Microbiology.  Rabbit.   PubMed:25344885 [IF=1.84] Wen, Zexing, et al. "Colony-stimulating factor 2 enhances the developmental competence of yak (Poephagus grunniens) preimplantation embryos by modulating the expression of heat shock protein 70 kDa 1A." Theriogenology (2017).  IF(ICC) ;  Rabbit.   PubMed:28257862
Immunogen Species	Goat
Clonality	Polyclonal
React Species	Rabbit,
Applications	IF=1:100-1000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Form	Liquid
Concentration	2.0 mg/ml
immunogen	Native rabbit IgG
Lsotype	IgG
Purification	affinity purified by Protein G, nonspecific adsorbed
Buffer Solution	10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
Storage	Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Product Detail	Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Product Picture	Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (SL0199R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF555) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated (SL0199R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF555) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (SL5960R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF555) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (SL5960R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF555) for 90 minutes, and DAPI for nuclei staining.

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